Fluorescent measurements of H+/K+ exchange in primary cultured inner medullary collecting duct cell monolayers of rabbit kidney / 中国药理学通报

ABSTRACT

AIM　To establish the fluorescent measurement technique for H+/K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney. METHODS　To measure H+/K+ exchange in the primary cultured IMCD cell monolayers under different CO2 concentrations by the BCECF/AM fluorescent probe.RESULTS　［pH］i recovery rate after acid loaded in the respiratory acidosis group was 0.0620±0.012，and was significantly higher than that of the control group（0.0504±0.009，P<0.05）;which in the respiratory alkalosis group was 0.0476±0.007, there was no signifacant difference between ALG and CG （P>0.05）. CONCLUSION　The fluorescent measurements of H+/K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney has many benefits which are convenient manipulation, stable and credit result, economy and safety , this method should further be popularized.