Quantitative detection of prostate specific membrane antigen and the splicing variant DNA in different tumor cell strains and prostate tissues / 中华老年医学杂志
Chinese Journal of Geriatrics
;
(12): 602-604, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-415567
ABSTRACT
Objective To understand and illuminate the bionomic characteristics of prostate specific membrane antigen (PSMA) and splicing variant PSMA5, through detecting the DNA levels of them in different tumor cell strains and prostate tissues. Methods The fluorescent quantization reverse transcriptase PCR (FQ-RT-PCR) method built up by our research group was used to detect the PSMA and variant PSMA5 DNA levels in different tumor cell strains and prostate tissues. Results The PSMA and PSMA5 DNA levels in tumor cell strains and pathological prostatic tissues were obviously more than those of the normal prostatic tissues (F=3.40, 11.94, both P<0.05), and the PSMA5 DNA level was much higher than was the PSMA DNA level in prostatic carcinoma tissues (P<0.05). Conclusions The different expressions between PSMA and PSMA5 in different tumor cells and prostatic tissues show that PSMA5 is more specific than PSMA as a prostate carcinoma tumor marker.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Geriatrics
Año:
2011
Tipo del documento:
Artículo
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