New Lung Cancer Panel for High-Throughput Targeted Resequencing
Genomics & Informatics
;
: 50-57, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-41695
ABSTRACT
We present a new next-generation sequencing-based method to identify somatic mutations of lung cancer. It is a comprehensive mutation profiling protocol to detect somatic mutations in 30 genes found frequently in lung adenocarcinoma. The total length of the target regions is 107 kb, and a capture assay was designed to cover 99% of it. This method exhibited about 97% mean coverage at 30x sequencing depth and 42% average specificity when sequencing of more than 3.25 Gb was carried out for the normal sample. We discovered 513 variations from targeted exome sequencing of lung cancer cells, which is 3.9-fold higher than in the normal sample. The variations in cancer cells included previously reported somatic mutations in the COSMIC database, such as variations in TP53, KRAS, and STK11 of sample H-23 and in EGFR of sample H-1650, especially with more than 1,000x coverage. Among the somatic mutations, up to 91% of single nucleotide polymorphisms from the two cancer samples were validated by DNA microarray-based genotyping. Our results demonstrated the feasibility of high-throughput mutation profiling with lung adenocarcinoma samples, and the profiling method can be used as a robust and effective protocol for somatic variant screening.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
Adenocarcinoma
/
Tamizaje Masivo
/
Sensibilidad y Especificidad
/
Polimorfismo de Nucleótido Simple
/
Secuenciación de Nucleótidos de Alto Rendimiento
/
Exoma
/
Pulmón
/
Neoplasias Pulmonares
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Genomics & Informatics
Año:
2014
Tipo del documento:
Artículo
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