Subcutaneous implantation of bone marrow mesenchymal stem cells-polyglycolic acid scaffold complex to construct small diameter tissue-engineered blood vessels / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 9544-9548, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-423918
ABSTRACT
BACKGROUND:
Our former studies have shown that bone marrow mesenchymal stem cells (BMMSCs) can be induced differentiation to vascular smooth muscle-like cells (VSMLCs) and vascular endothelium-like cells (VELCs), which are compatible with collagen-embedded polyglycolic acid scaffolds.OBJECTIVE:
To investigate the possibility of constructing small diameter tissue-engineered blood vessels via subcutaneous implantation.METHODS:
The cells-scaffold complex was produced by separately seeding VSMLCs and VELCs derived from BMMSCs on polyglycolic acid collagen scaffolds. The two layers were separated by ECMgel. The cells-scaffold complex was subcutaneous implanted into small diameter tissue-engineered blood vessels.RESULTS ANDCONCLUSION:
Histological analysis of the small diameter tissue-engineered blood vessel walls revealed a typical artery structure, which was similar to natural vessels. The tissue-engineered blood vessels were not broken down under a force of 26.6 kPa. Eight weeks after implantation, the Brdu-labeled seed cells were found in the three layers of the vessel walls. The results revealed that the subcutaneous tissue was a good bioreactor to construct small diameter tissue-engineered blood vessels.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2011
Tipo del documento:
Artículo
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