Effect of hydroxysafflor yellow A on neurogenesis in a rat model of oxygen-glucose deprivation in hippocampal slice cultures / 国际脑血管病杂志

ABSTRACT

Objective To investigate the effect of hydroxysafflor yellow A (HSYA) on neurogenesis of the dentate gyrus organotypic hippocampal slice cultures after oxygen-glucose deprivation (OGD).Methods The 3-day-old Sprague Dawley rats were used to making organotypic hippocampal slices.They were randomly divided into four groupsa blank control group,a saline control group (giving 1 mL normal saline after OGD),a high-dose HSYA group (giving 1 ml,0.072 mg/ml HSYA after OGD),and a low-dose HSYA group (giving 1 ml,0.036 mg/ml HSYA after OGD) (n =6 slices in each group).Nestin and 5-bromo deoxyuridine nucleoside imnune fluorescence method were used to double-label the proliferation of neural stem cell (NSC).Results There were significant differences in NSC numbers among the different time points before and after OGD in slices of each group (all P ＜0.001).After OGD,the proliferation of NSC numbers reduced significantly in each group,cell proliferation increased siginificantly at day 3,and the proliferation of NSC number at day 6 was lower than that at day 3,but they still higher than those before OGD.There were significant differences in the proliferation of NSC numbers among the blank control,the saline control,high-dose HSYA,and low-dose HSYA groups (F =947.077,P ＜0.001).Observing from each time point,in addition to before OGD,the proliferation of NSC numbers at all other time points had the following relationsthe blank control Group ＞ the high-dose HSYA group ＞ the low-dose HSYA group ＞ the saline control group (all P＜0.001).Conclusions HSYA may alleviate OGD to the NSC injury and contribute to neurogenesis.