Simultaneous determination of rutin and naringellin-7-O-glucoside in extraction of Lysimachia clethroide Duby by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12): 106-109, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-433188
ABSTRACT
AIM:
To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS:
Using Diamonsil C_(18) (4.6 mm×250 mm,5 μm) as analytical column.The mobile phase consisted of acetonitrile (A) and 0.1% phosphoric acid ( B ) with gradient elution 0~18rain,83%~80% B; 18~30 min,80%~86% B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min; Column temperature was at 35 ℃.RESULTS:
A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00~48.00 μg/mL( r =0.999 3 ) and 0.64 ~ 40.72 μg/mL( r = 0.999 8 ),respectively.CONCLUSION:
The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Traditional Patent Medicine
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS