The influence of PTD-OD-HA fusion proteins on apoptosis of bcr/abl positive cell lines / 肿瘤
Tumor
;
(12): 267-271, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-433358
ABSTRACT
Objective:
To study the influence of protein transduction domain (PTD)-oligomerization domain (OD)-HA fusion proteins on apoptosis of bcr/abl-positive cell lines.Methods:
bcr/abl-positive cells were treated with PTD-OD-HA protein. The apoptoses of the cells were detected by flow cytometry (FCM), DNA ladder and transmission electron microscopy (TEM), and the levels of apoptosis-related genes bax and bcl-2 were detected by RT-PCR and Western blotting.Results:
FCM examination demonstrated that PTD-OD-HA protein induced the apoptosis of bcr/abl-positive cells; DNA ladder showed that the classic DNA ladders appeared in BaF3-P210 and K562 cells after 48 h treatment with PTD-OD-HA proteins; the apoptoses of BaF3-P210 cells were observed by TEM; the levels of bax in mRNA and protein increased in BaF3-P210 and K562 cells, and bcl-2 decreased.Conclusion:
PTD-OD-HA proteins specifically induced the apoptosis of bcr/abl positive cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Tumor
Año:
2010
Tipo del documento:
Artículo
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