Human Immunodeficiency Virus-Infected T Cells Are Selectively Killed by Monoclonal Anti-gp120 Antibody Coupled to Pokeweed Antiviral Protein
Journal of the Korean Society of Virology
;
: 383-391, 1998.
Artículo
en Coreano
| WPRIM
| ID: wpr-43336
ABSTRACT
A murime monoclonal antibody (mAb) specific for the envelope glycoprotein gp120 of human immunodeficiency virus type-I (HIV-1) was chemically coupled to pokeweed antiviral protein (PAP) from Phytolacca americana. The immunotoxin was purified by FPLC using 5200 colum. The purified immunotoxin efficiently bound to HIV-infected T cells as evidenced by fluorescence-activated cell sorter analysis. The immunotoxin selectively killed human T lymphoid lines infected with HIV-lIIIB at less than 250 pM of the immunotoxin cells, while PAP or mAb alone did not have any significant effect on infected cells. The uninfected control T cell lines were not affected. Human cells infected with HIV-2 or other HIV-1 strains were not killed, suggesting that the killing depends completely on the antibody used for coupling. These in vitro results suggest that the PAP-mAb conjugate may be used to selectively remove cells expressing viral antigens from individuals infected with HIV.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Glicoproteínas
/
Linfocitos T
/
Línea Celular
/
Inmunotoxinas
/
VIH-1
/
VIH
/
VIH-2
/
Phytolacca americana
/
Homicidio
/
Antígenos Virales
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Society of Virology
Año:
1998
Tipo del documento:
Artículo
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