Your browser doesn't support javascript.
loading
Human Immunodeficiency Virus-Infected T Cells Are Selectively Killed by Monoclonal Anti-gp120 Antibody Coupled to Pokeweed Antiviral Protein
Journal of the Korean Society of Virology ; : 383-391, 1998.
Artículo en Coreano | WPRIM | ID: wpr-43336
ABSTRACT
A murime monoclonal antibody (mAb) specific for the envelope glycoprotein gp120 of human immunodeficiency virus type-I (HIV-1) was chemically coupled to pokeweed antiviral protein (PAP) from Phytolacca americana. The immunotoxin was purified by FPLC using 5200 colum. The purified immunotoxin efficiently bound to HIV-infected T cells as evidenced by fluorescence-activated cell sorter analysis. The immunotoxin selectively killed human T lymphoid lines infected with HIV-lIIIB at less than 250 pM of the immunotoxin cells, while PAP or mAb alone did not have any significant effect on infected cells. The uninfected control T cell lines were not affected. Human cells infected with HIV-2 or other HIV-1 strains were not killed, suggesting that the killing depends completely on the antibody used for coupling. These in vitro results suggest that the PAP-mAb conjugate may be used to selectively remove cells expressing viral antigens from individuals infected with HIV.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Glicoproteínas / Linfocitos T / Línea Celular / Inmunotoxinas / VIH-1 / VIH / VIH-2 / Phytolacca americana / Homicidio / Antígenos Virales Límite: Humanos Idioma: Coreano Revista: Journal of the Korean Society of Virology Año: 1998 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Glicoproteínas / Linfocitos T / Línea Celular / Inmunotoxinas / VIH-1 / VIH / VIH-2 / Phytolacca americana / Homicidio / Antígenos Virales Límite: Humanos Idioma: Coreano Revista: Journal of the Korean Society of Virology Año: 1998 Tipo del documento: Artículo