Construction and Enzyme-activity Assessment of L-Asparaginase Mutants / 中国药科大学学报
Journal of China Pharmaceutical University
;
(6): 468-472, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-434051
ABSTRACT
AIM:
To construct nine novel L-asparaginase mutants and study their enzyme-activity.METHODS:
The mutants were constructed using overlap extension PCR according to the principle of alanine-scanning mutagenesis. The enzyme-activity was detected by Nessler's method.RESULTS:
The DNA sequencing showed that the mutagenesis was consistent with the theoretical prediction. The enzyme-activity assay demonstrated that each mutant possessed enzyme activity equal to the original enzyme.CONCLUSION:
Through gene modification,epitop of L-asparaginase was changed without activity loss.These results provide foundation for further study of the structure-function relationship of L-asparaginase.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of China Pharmaceutical University
Año:
2005
Tipo del documento:
Artículo
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