Changes of renal histology and expression of IFN-γ of normobaric hyperoxia in neonatal rats / 国际儿科学杂志

ABSTRACT

Objective To study changes in renal histology of neonatal rats with prolonged hyperoxic lung injury and monitored the expression of IFN-γ in kidney.Methods Full-time newborn rats were continuously exposed to oxygen(80％ ±5％ 02,n =100) or room air(21％ O2,n =100) after birth.Dynamic changes of renal histology and the expression of interferon (IFN)-γin kidney by immunohistochemical method were monitored on day 1,3,7,14,21 in the hyperoxia and control group.Results Normobaric hyperoxia indnced mild renal damage including renal tubular hydropic degeneration,edema,dilation,tubular interstitial vessel dilation,congestion,and rare tubular hemorrhage,necrosis,regeneration.Fibrosis were not seen.On day 3,7,14,the intensity of IFN-γexpression increased compared with the control group (P ＜ 0.05) and significantly increased on day 7 (P ＜ 0.01).IFN-γwere mainly expressed in tubular epithelia cells.On day 21,the intensity of IFN-γ expression decreased,and there was little difference compared with the control group.Condusions Renal damage can be induced by prolonged hyperoxia exposure.The intensity of IFN-γexpression increased in kidney,which contributed to the damage of kidney.