Survivin antisense oligonucleotide induces apoptosis of SGC7901 cells and enhances sensitivity to taxol / 临床与实验病理学杂志

ABSTRACT

Purpose To investigate the anti-tumor effect of survivin antisense oligonucleotide (Asodn),and whether it can strengthen the sensitivity of chemical therapy with taxol.Methods The experiment was devided into four groups:control group,liposome group,sense oligonucleotide group,and antisense oligonucleotide group.Survivin antisense oligonucleotide was synthesized,and transfected into gastric cancer cells with liposome.The inhibitory rate of proliferation was tested with MTT,and the expression of survivin protein with Western blot;the morphological changes of apoptosis through Hoechst staining were observed, and the apoptosis rate with flow cytometer.Results As observed through Hoechst staining, the cancer cells had normal blue nucleus in the control group, Lip group, and Sodn group, while in Asodn transfection group the nucleus became condensed, with karyorrhexis. The cell inhibitoty rate in Asodn group increased, presenting in a time-dosage dependence manner; survivin protein expression reduced and apoptotic rate increased; there were differences in statistical significance (P<0.05), as compared with the control group, Lip group and Sodn group. The proliferation-inhibitory rate in Asodn plus taxol group [(78.1±0.8) %] was obviously higher than that in Asodn group [(54.9±1.6)%] and taxol group [(56.7%±0.7)%] (P<0.05).Conclusion Survivin antisense oligonucleotide can inhibit the proliferation of gastric cancer cells, induce apoptosis, and strengthen the effect of taxol on the inhibition of tumor growth.