Promoter methylation of RASSF1A gene in hepatocellular carcinoma and its clinical significance / 中华普通外科杂志
Chinese Journal of General Surgery
;
(12): 300-303, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-436128
ABSTRACT
Objective To explore the relationship between promoter methylation of RASSF1 A gene and clinico-pathological characteristics in hepatocellular carcinoma.Methods MS-PCR was used for analyzing the status of aberrant promoter methylation of RASSF1A in 100 primary HCC samples and adjacent noncancerous tissues,10 normal liver tissues,and six HCC cell lines.RT-PCR was used to assess reactivation of RASSF1A expression after HCC cell lines treated with demethylating agent 5'-aza-2' deoxycytideing.Results Abnormal promoter methylation of RASSF1A gene was found in 69(69%) cases of HCC,15 (15%) cases of adjacent normal tissues,and no abnormal promoter methylation of RASSF1A gene was found in normal liver tissues,and the difference was statistically significant (x2 =67.75,P <0.001).The methylation of RASSF1A gene was correlated to HBsAg (x2 =11.341,P < 0.05) and histological differentiation(x2 =10.575,P < 0.05).Four cell lines with abnormal CpG island methylation of RASSF1A gene were all re-expressed after treated with 5'-Aza-CdR.Conclusions RASSF1A gene promoter methylation was correlated to HBsAg and histological differentiation and this is one of the most important mechanism for low expression of RASSF1A in HCC.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of General Surgery
Año:
2013
Tipo del documento:
Artículo
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