Caveolin-1 inhibits vascular anastomotic stenosis and down regulates TNF-α expression / 中华普通外科杂志
Chinese Journal of General Surgery
;
(12): 624-627, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-437002
ABSTRACT
Objective To investigate the effect of caveolin-1 on rabbit carotid artery anastomotic stenosis and its relationship with TNF-α.Methods 40 New Zealand white rabbits were randomly divided into normal group,surgical group,empty vector group and caveolin-1 transfecting group.Carotid artery end-to-end anastomosis was done in the rabbits except these in normal group.Five specimens were randomly taken on day 7 after surgery for Westem blot to detect the expression of caveolin-1 and TNF-α; The rest specimens were taken for HE staining at fourth week.The ratio of intima/media area were measured by Image-Pro Plus 6.0 software in order to observe the proliferation of intima.Results Compared with normal group,in surgical group intimal proliferation was significant,the intima/media ratio was significantly higher (P < 0.05) ; Compared with surgical group,in caveolin-1 transfected group neointimal proliferation was not obvious,the intima/media ratio decreased (P < 0.05).Western blot showed thatcompared with the surgical group,caveolin-1 expression in transfected group was significantly higher (P < 0.05) ; compared with normal group,the TNF-α expression in surgical group increased (t41.28,P < 0.05) ; Compared with surgical group,TNF-α expression in transfected group decreased significantly (t36.37,P < 0.05).Conclusions Caveolin-1 inhibits vascular anastomotic stenosis,possibly by down-regulating TNF-α expression.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of General Surgery
Año:
2013
Tipo del documento:
Artículo
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