Effects of enamel matrix derivatives on biologic activities of human periodontal fibloblasts to demineralized root surface / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 679-690, 2008.
Artículo
en Coreano
| WPRIM
| ID: wpr-43835
ABSTRACT
PURPOSE:
The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). MATERIAL ANDMETHODS:
Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and 100 microgram/ml concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done.RESULTS:
Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days.CONCLUSION:
Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ligamento Periodontal
/
Tetraciclina
/
Diente
/
Recuento de Células
/
Ácido Edético
/
Esmalte Dental
/
Dentina
/
Electrónica
/
Electrones
/
Fosfatasa Alcalina
Límite:
Humanos
Idioma:
Coreano
Revista:
The Journal of the Korean Academy of Periodontology
Año:
2008
Tipo del documento:
Artículo
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