Bone marrow mesenchymal stem cells against monocrotaline-induced pulmonary artery hypertension** / 中国组织工程研究

ABSTRACT

BACKGROUND:Stem cel transplantation has a certain effect in the treatment pulmonary arterial hypertension. OBJECTIVE:To investigate the effect of bone marrow mesenchymal stem cel s transplantation on the treatment of pulmonary arterial hypertension and to discuss the mechanism. METHODS:Bone marrow mesenchymal stem cel s were in vitro cultured, purified and amplified by density gradient centrifugation method, and labeled with the fluorescent dye for preparation. Pulmonary arterial hypertension model was established by subcutaneous injection of monocrotaline. One week after modeling, the rats were randomly divided into three groups. Rats in the stem cel transplantation group and pulmonary arterial hypertension group received subcutaneous injection of monocrotaline to establish the pulmonary arterial hypertension model. One week later, the rats in the stem cel transplantation group received sublingual vein injection of bone marrow mesenchymal stem cel solution, the rats in the pulmonary arterial hypertension group were injected with the culture medium without stem cel s, and the rats in the control group were injected with the normal saline in the same dose. RESULTS AND CONCLUSION:At 2 weeks after transplantation, compared with the mesenchymal-induced pulmonary arterial hypertension rats, the hemodynamic parameters and the ratio of right ventricular/body weight of the rats in the stem cel transplantation group were significantly improved (P<0.05);the degree of pulmonary vascular remodeling was significantly reduced (P<0.05). Fluorescence microscope observation showed that the transplanted bone marrow mesenchymal stem cel s could alive at least 2 weeks in the stem cel transplantation group, and part of the stem cel s could differentiate into pulmonary vascular endothelial cel s. The results show that bone marrow mesenchymal stem cel transplantation can significantly improve the pulmonary vascular and right ventricular structural impairments in the rats with mesenchymal-induced pulmonary arterial hypertension.