Evaluation of a New Immunochromatographic Assay Kit for the Rapid Detection of Norovirus in Fecal Specimens
Annals of Laboratory Medicine
; : 79-81, 2012.
Article
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| WPRIM
| ID: wpr-43982
Biblioteca responsable:
WPRO
ABSTRACT
Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Juego de Reactivos para Diagnóstico
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ARN Viral
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Inmunoensayo
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Enfermedad Aguda
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Sensibilidad y Especificidad
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Infecciones por Caliciviridae
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Norovirus
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Heces
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Gastroenteritis
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Child, preschool
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Humans
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Infant
Idioma:
En
Revista:
Annals of Laboratory Medicine
Año:
2012
Tipo del documento:
Article