Content Determination of Alkaloids in Keke Tablets by HPLC / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
; (12): 53-55, 2013.
Article
en Zh
| WPRIM
| ID: wpr-441428
Biblioteca responsable:
WPRO
ABSTRACT
Objective To establish the method for determining ephedrine hydochloride and pseudoephedrine hydrochloride in Keke Tablets by HPLC. Methods The samples were analyzed by a phemomenex Synergi Polar-RP column (4.6 mm×250 mm, 4 μm), with mobile phase methanol∶0.092%phosphoric acid (0.04%triethylamine and 0.02%second butylamine)=1.5∶98.5 at flow rate of 1.0 mL/min and detection at UV wavelength of 210 nm. The column temperature was 30 ℃. Results The linear ranges of ephedrine hydrochloride and pseudoephedrine hydrochloride were 6.51×10-3-0.651 μg (r=0.999 9) and 6.27×10-3-0.627 μg (r=1), respectively. The average recoveries (n=6) of ephedrine hydrochloride and pseudoephedrine hydrochloride were 102.26%and 103.71%, with RSD of 0.34%and 0.22%, respectively. Conclusion This method is simple, accurate, reproducible, highly specific and reliable results, and has the ability to effectively control the quality of alkaloids in Keke Tablets.
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WPRIM
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Zh
Revista:
Chinese Journal of Information on Traditional Chinese Medicine
Año:
2013
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Article