Study about effects of BCG treatment on expression of stem cell factor in asthmatic mice / 临床儿科杂志

ABSTRACT

Objective To study the effects of Bacillus Calmette-Guerin (BCG) intervention on the expression of stem cell factor (SCF) in asthma. Methods Kunming mice were randomly divided into asthmatic group, BCG group and control group of ten mice each group. Mice were sensitized and challenged with ovalbumin (OVA) to establish asthmatic model. After twenty-four hours of last challenge, eosinophils (EOS) were counted in the lung tissue and bronchoaveolar lavage fluid (BALF). The level of SCF in BALF was determined by enzyme-linked immunosorbent assay. The expression of SCF protein in lung tis-sue was measured by immunohistochemistry technique and computerized image analysis system. Results The number of EOS in lung tissue was (10.67±1.94)/HP, (6.40±1.55)/HP and (0.37±0.33)/HP in asthmatic, BCG and control group respectively. The number of EOS in BALF was (7.58 ± 1.30) × 107/L, (3.78 ± 1.15) × 107/L and 0 in asthmatic, BCG and control group respectively. The difference between each group was statistically significant (P0.05). In asth-matic group, the expression of SCF in lung was positively correlated with the number of EOS (P<0.05). Conclusions BCG treat-ment can markedly decrease the airway inflammation in asthmatic mice. BCG cannot inhibit the expression of SCF in asthma.