Exclusion of an outbreak of human brucellosis / 中华地方病学杂志

ABSTRACT

Objective To prevent misdiagnoses of brucellosis and misjudgment of brucellosis outbreak through differential diagnosis between influenza and brucellosis of three new cases who were in the same work unit and underwent the longest latent period.Methods Contact history of the three new cases was investigated epidemiologically and physical examination and blood test were taken.Brucellosis antibodies of the cases and abortion cow were tested by serum tube agglutination test (SAT),and pathogen was isolated.The tonsilla and pharyngeal secretion substance of cases were collected for detection of influenza virus RNA by real-time PCR.Results The cases had contact history with brucellosis abortion cattle and/or the animal contaminated environment,and had a fever,fatigue and other symptoms.Total blood cell(WBC) was normal and proportion of lymphocyte was increased.The antibody titer in SAT test was 1200(+++),but pathogen isolation was negative for all three cases.Brucellosis antibody of abortion cow was positive and antibody titer was 1800 (++).The test of Bv RNA of influenza virus was positive in the three cases.Conclusions The three cases are brucellosis latent infection combined with influenza and we have excluded a misjudgment of pestilence of human brucellosis outbreak.