The effect of AGEs on NADPH oxidase expression in the process of islet injuring / 实用医学杂志

ABSTRACT

Objective To investigate the effects of advanced glycation end products (AGEs) on NADPH oxidase expression in rats′ pancreatic cells. Methods Isets of rats were isolated and intervened with AGEs for certain times (2, 12, 24, 48 h). The Nox2 mRNA expression was assayed by reverse transcription polymerase chain reaction (RT-PCR) and Real-time PCR. NADPH oxidase activity was detected by lucigenin-enhanced chemiluminescence. Results Nox2 mRNA level was 1.75, 2.31-fold higher in AGEs intervened group than control at 12 h and 24 h respectively. The NADPH oxidase activity was increased by AGEs stimulation in a time dependent manner. A distinct peak was at 24 hours and then with a decline , which is still higher than that of the control. Conclusion Exposure of rat islet to AGEs induces NADPH oxidase expression and leads to injury of islet.