CCL18 for promoting breast cancer SK-3rd cells invasion via integrin aggregation / 重庆医学

ABSTRACT

Objective To explore the role of integrin in CCL18 for promoting breast cancer SK-3 rd cells invasion and migration process to illuminate the molecular mechanism of CCL 18 for promoting breast cancer SK-3 rd cells invasion and migration process . Methods The flow cytometry was adopted to detect CCL18-induced integrin aggregation ;Western blot was used to detect the focal adhesion kinase(FAK ) activation ,the infiltrating migrationin experiment was adopted to determine the invasion and migration of SK-3rd cells and the siRNAs transfection was used to detect the expression of silence integrin β1 .Results CCL18 promoted the in-tegrinβ1 aggregation in breast cancer SK-3rd cell surface and further promoted the integrin-mediated phosphorylated activation of FAK .Under the reaction of CCL18 ,the cells number of SK-3rd cellular invasion and migration was increased by ten times (P<0 .01) ,which was obviously decreased by siRNA silenced integrin β1 .Conclusion CCL18 promotes breast cancer invasion and mi-gration via integrin aggregation .