Effect of Purpura Decoction and Its Disassembled Prescriptions on Reticulated Platelet and ;Thrombopoietin of Thrombocytopenic Purpura Model Mice / 中国中医药信息杂志

ABSTRACT

Objective To observe the effects of Purpura decoction and its disassembled prescriptions on peripheral blood reticulated platelets (RP) and serum thrombopoietin (TPO) of idiopathic thrombocytopenic purpura (ITP) model mice, to explore the action mechanism and core structure of the prescription. Methods Totally 84 BalB/c mice were randomly divided into normal group, model group, prednisone group, Purpura decoction group, Buyi Qixue group, Buqi Huoxue group and Buxue Huoxue group. ITP mouse model was made by injecting intraperitoneally guinea pig-antimouse platelet serum. From the 5th day of modeling, mice were intragastrically administered. The normal group and model group were fed with normal saline 0.2 mL per mouse. Purpura decoction group was fed with Purpura decoction 0.43 mL per mouse. Buyi Qixue group was fed with Buyi Qixue decoction 0.36 mL per mouse. Buqi Huoxue group was fed with Buqi Huoxue decoction 0.27 mL per mouse. Buxue Huoxue group was fed with Buxue Huoxue decoction 0.3 mL per mouse. Prednisone group was fed with prednisone acetate suspension 0.2 mL per mouse. Ten days later, took the blood from the eyeball and isolated serum, the mouse peripheral platelet, RP and TPO were observed. Results After treatment, the platelet count of treatment groups was improved. Compared with before treatment, Purpura decoction group,Buqi Huoxue group and prednisone group had significant difference in platelet count (P<0.05). The RP of model group was significantly higher than the normal group (P<0.05), but RP absolute value was lower than the normal group (P<0.05). Compared with the model group, RP of treatment groups reduced (P<0.05), and RP was negatively correlated with platelet count (P<0.05). TPO level of the model group was higher than that of the normal group (P<0.01). TPO of treatment groups declined significantly, and had significant differences with that of the model group (P<0.05). Conclusion Core structure of Purpura decoction is qi tonifying and blood activating drug. The mechanism of treating ITP is related with lowering RP and TPO.