Molecular Cloning and Characterization of HCT (Hydroxycinnamoyl-CoA:Shikiate/Quinate Hydroxycinnamoyltransferase) Gene in Lonicera japonica Thunb / 世界科学技术-中医药现代化

ABSTRACT

Hydroxycinnamoyl-CoAshikimate/quinate hydroxycinnamoyltransferase(HCT) is an key enzyme involved in the biosynthesis of chlorogenic acid in Lonicera japonica. In this study, eight putative HCT genes were cloned with RACE (rapid amplification of cDNA ends) technology based on the analysis of transcriptome in L. japonica. Among them, one was suggested as HCT gene (LjHCT) in L. japonica through analysis of sequence similarity, physical and chemical properties, and domain conservation of the proptein. LjHCT gene containing 1 275 bp encodes a protein with the molecular weight of 47 kDa. These results will provide foundation for exploring the function of LjHCT in Lonicera japonica.