Antimicrobial and sealant properties of nanohydroxyapatite as endodontic sealer / 中国组织工程研究

ABSTRACT

BACKGROUND:Depending on different pH values, nanohydroxyapatite materials have different purities, whether root canal sealers formed by which exert effects on antimicrobial resistance, root canal closure and apical histocompatibility has no reports. OBJECTIVE:To compare the antimicrobial and sealant properties of nanohydroxyapatite materials with different pH, nanohydroxyapatite-composite and traditional root canal sealants. METHODS:We detected the antimicrobial action of nanohydroxyapatite with pH 8, 9, 10, Vitapex and AH-Plus root canal sealants with and without addition of ornidazole against three microbial strains namely Enterococcus faecalis, Candida albicans and Staphylococcus aureus using agar diffusion method. And we also analyzed the endodontic microleakage of six root canal sealants by determining the apical reservoir glucose concentration using Glucose Oxidase Method. RESULTS AND CONCLUSION:Pure nanohydroxyapatite with different pH did not show antimicrobial properties. Addition of ornidazole to nanohydroxyapatite showed greater inhibitory action against Enterococcus faecalis, lesser in Staphylococcus aureus, fol owed by Candida albicans. Vitapex root canal sealer had inhibitory effects only against Staphylococcus aureus. AH-Plus, itself, had antimicrobial activity against al the three strains, but the antimicrobial activity decreased after addition of ornidazole. Nanohydroxyapatite, as a root canal sealant, was superior to zinc oxide eugenol and Vitapex, but inferior to AH-Plus. Addition of ornidazole to nanohydroxyapatite for a short period showed no impact on sealant properties of the material.