Immune response of purified Plasmodium falciparum antigen on human pe-ripheral blood T lymphocytes in vitro / 中国血吸虫病防治杂志

ABSTRACT

Objective To explore the effects of the purified Plasmodium falciparum(P. f)antigen on the T lymphocytes of the peripheral blood of healthy people in vitro. Methods The peripheral blood mononuclear cells(PBMCs)isolated from healthy donators were cultured with purified P. f antigen(5μg/ml)and normal RBC(nRBC)antigen(5μg/ml),in addition, the group in which only IL-2 added was set as a negative control. After cultured for 12 d,the corresponding antigens were used to re-stimulated the activated T cells in the two stimulated groups for 20 h. Then the cells were collected,the proliferations of the T cells labeled by CFSE were analyzed,and the secretion condition of IL-4 and IFN-γof the cells were detected by flow cytome-try. Results After amplification,the proliferative index(PI )of CD4+T cells that were stimulated with P. f antigen was signifi-cantly higher than those in the nRBC antigen group and the negative control group(both P0.05). Meanwhile,the percentages of the CD4+T cells se-creting IL-4+in the P. f antigen group was significantly higher than those in the other two groups(both P0.05). Conclusion The purified P. f anti-gen could effectively stimulate the proliferation of CD4+T cells in peripheral blood of healthy people,and the latter can play a role in immunoregulation through secreting IL-4 preferentially.