Application of Fluorescence Quantitative PCR in Salmonella Rapid Detection in Drugs / 中国药师
China Pharmacist
;
(12): 242-244, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-452779
ABSTRACT
Objective:
To establish a Taqman fluorescence quantitative PCR for the detection of Salmonella in drugs.Methods:
Based on Salmonella specific gene, a pair of primers and a probe were designed, and DNA of Salmonella was extracted for the detec-tion.Results:
The method was much specific, and the detection limit was 160 cfu/ml. The detection rate was 100% for the artificially contaminated samples.Conclusion:
Fluorescence quantitative PCR can be applied in the rapid detection of Salmonella in drugs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
China Pharmacist
Año:
2014
Tipo del documento:
Artículo
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