Correlation of Cavernous Relaxation with Nitric Oxide Concentration in the Rabbit / 대한비뇨기과학회지

ABSTRACT

Nitric oxide (NO) is known to act as an important neural mediator of penile erection. However, the degree of penile erection related to the concentration of NO released from corpus cavernosum has not been known yet. The present study was undertaken to correlate the degree of relaxation of the corpus cavernosum with concentration of NO by treatment of various NO releasing agents. Isometric tension of the rabbit cavernous strips was measured by polygraph system. The concentration of NO released from the same strips was simultaneously measured using an electrochemical method (Iso NO meter), which allows to detect change of NO gas level in perfusate of the organ bath. The cumulative additions of endothelial dependent agents, both acetylcholine and bradykinin at concentration from 0.00000001 to 0.0001M relaxed the cavernous strips precontracted by 0.000001M phenylephrine in concentration-dependent manner, which were highly correlated with the concentration of NO (38.9 +/- 15.2 nM at 0.00000001M - 74.5 +/- 18.4 nM at 0.0001M of acetylcholine; 30.2 +/- 5.8 nM at 0.00000001M - 90.5 +/- 10.2 at 0.0001M of bradykinin) released from the same strips, Furthermore, 3-[(3-cholamido propyl)]-l-propane sulfonate (CHAPS), a deendothelial agent, markedly suppressed both acetylcholine or bradykinin-induced cavernous relaxation and abolished NO release. In contrast, endothelial independent agent such as sodium nitroprusside (SNP), N-ethoxycarbonyl-3-morpholino-sydonimine (SIN-l) and s-nitroso-N-acetylpenicillamine (SNAP) at concentration from 0.00000001 to 0.001M relaxed the cavernous strips in` concentration dependent manner, without altering the basal concentration of NO in perfusate. From these results, it appears that the degree of cavernous relaxation induced by acetylcholine or bradykinin is highly correlated with the concentration of NO released from the cavernous endothelium. Furthermore, the direct electrochemical measurement of NO concentration in perfusate may be useful for further NO research in association with penile erection.