Quantitative Determination of Sildenafil in Aphrodisiacs by LC-MS / 医药导报
Herald of Medicine
;
(12): 1373-1375, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-454546
ABSTRACT
Objective To establish a method for the quantitative determination of sildenafil in aphrodisiacs by liquid chromatography-mass spectrometry (LC-MS). Methods Chromatography was carried on a Waters BEH C18 column (50 mmí2. 1 mm,1. 7 μm) with the column temperature being 35 ℃ and 0. 02 mol·L-1 ammonium acetate water solution (containing 0. 1% acetic acid)-acetonitril (7030) as the mobile phase at a flow rate of 0. 2 mL·min-1 . Determination was performed by multiple reaction monitoring mode in two channels,475→100,475→283,using electrospray ionization in positive ion mode. Results Sildenafil had a good linear relationship in the range of 0. 1986-1. 986 ng with the linear regression equation being Y=27 750X+15 232 (r=0. 995 2). The average recovery was 96. 6% with RSD being 1. 8% (n=9). The limits of quantitation and detection were 0. 04 ng and 0. 01 ng,respectively. Conclusion The determination results of 10 batches of samples show that the method is sensitive and accurate and can be used as an appropriate technique to detect sildenafil in aphrodisiacs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Herald of Medicine
Año:
2014
Tipo del documento:
Artículo
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