Development of a time-resolved fluorescence immunoassay for rapid determinat-ion of levels of cardiac troponin Ⅰantigen in human serum / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1093-1097, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-454850
ABSTRACT
To establish a method to detect cardiac troponin I by using time-resolved fluoroimmunoassay ( TRFIA) and apply to the clinic.Methods:
The assay were measured by TRIFA and double antibody sandwich method .Standard protocols were evaluated with the standard curve , the limit of detection , stability, precision and cross reaction .Healthy reference populations and clinical serum specimens were measured to established the reference interval and evaluated the perspective of the clinical application .Results:
The standard curve was Y=7485 .878+1400.924 X with a correlation coefficient of 0.999.The limit of detection was 0.052 ng/ml.The intra-and inter-assay coefficients of variation ( CV) were all 0.05 ).There was significant difference in statistics compared before and after treatment with AMI ( P<0.001 ) .Conclusion:
The TRFIA method for detecting cTnI achieves clinical application standards and may be used for the diagnosis and serosurveillance of acute myocardial infarction patients.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Guía de Práctica Clínica
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
2014
Tipo del documento:
Artículo
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