Application of matrix-assisted laser desorption ionization-time of flight mass spectrometer in identification of clinical common bacteria / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 2228-2230, 2014.
Article
en Zh
| WPRIM
| ID: wpr-456064
Biblioteca responsable:
WPRO
ABSTRACT
Objective To evaluated the application value of two kinds of mass spectrometer(MS)and Vitek MS system in the i-dentification of routinely isolated bacteria in clinic.Methods 149 strains of common bacteria(including 14 genera and 30 species)i-solated from blood,urine,cerebral spinal fluid,secretion and sputum samples in our hospital from March 2012 to January 2013 were collected and simultaneously identified by 2 kinds of matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALD-TOF-MS).The identification results were compared with those identified by the conventional biochemical identification (Vitek2 compact).The strains with the inconsistent results identified by 3 kinds of method were confirmed by 16S rDNA gene se-quencing.Results Among 149 common bacteria,the correct identification rates of genus and species by the Bruker Biotyper MS were 98% and 96% respectively and which by the Vitek MS system were 97% and 95% respectively.There were no misidentified bacterial strains by these two kinds of MS.Conclusion No statistical difference in the identification results was observed between these two kinds of MS system(P >0.05).Both exhibit excellent identification level and are suitable for the routine laboratory iden-tification of clinical microorganism.
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WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
International Journal of Laboratory Medicine
Año:
2014
Tipo del documento:
Article