Simultaneous Determination of Protocatechualdehyde, Rosmarinic Acid and Salvianolic Acid A in Salvia Miltiorrhiza Bunge by HPLC / 中国药师
China Pharmacist
;
(12): 1473-1475, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-456307
ABSTRACT
Objective:
To establish an HPLC method to simultaneously determine the content of protocatechualdehyde, rosmarinic acid and salvianolic acid A in Salvia miltiorrhiza Bunge.Methods:
The chromatographic column was a Linksil-ODS ( 250 mm × 4. 6 mm,5 μm) column,the mobile phase was 1% acetic acid water solution-acetonitrile (7∶3) with the flow rate of 0. 8 ml·min-1 . The detection wavelength was 280nm , the column temperature was 30℃ and the injection volume was 20 μl.Results:
The linear range of protocatechualdehyde, rosmarinicacid and salvianolic acid A was 3-300 μg·ml-1(r>0. 999 0), and the recovery was within the range of 95. 22%-99. 68% with RSD below 2% (n=5).Conclusion:
The method is accurate, rapid and reproducible, and can be used to control the quality of Salvia miltiorrhiza Bunge.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacist
Año:
2014
Tipo del documento:
Artículo
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