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Molecular identification in genus of Lilium based on DNA barcoding / 药学学报
Yao Xue Xue Bao ; (12): 1730-8, 2014.
Article en Zh | WPRIM | ID: wpr-457277
Biblioteca responsable: WPRO
ABSTRACT
To establish a new method for identifying genus of Lilium by DNA barcoding technology, ITS, ITS2, psbA-trnH, matK and rbcL sequences were analyzed in term of variation of inter- and intra-species, barcoding gap, neighbor-joining tree to distinguish genus of Lilium based on 978 sequences from experimental and GenBank database, and identification efficiency was evaluated by Nearest distance and BLAST1 methods. The results showed that DNA barcoding could identify different species in genus of Lilium. ITS sequence performed higher identification efficiency, and had significant difference between intra- and inter-species. And NJ tree could also divide species into different clades. Results indicate that DNA barcoding can identify genus of Lilium accurately. ITS sequence can be the optimal barcode to identify species of Lilium.
Texto completo: 1 Índice: WPRIM Tipo de estudio: Diagnostic_studies Idioma: Zh Revista: Yao Xue Xue Bao Año: 2014 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Tipo de estudio: Diagnostic_studies Idioma: Zh Revista: Yao Xue Xue Bao Año: 2014 Tipo del documento: Article