Culture and identification of SK-N-SH neuroblastoma stem cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 7260-7265, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-457395
ABSTRACT
BACKGROUND:
Neuroblastoma is a common solid tumor in children. Pediatric tumors are little affected by environmental factors, but closely related to child development. The suspension method is an effective and reliable method to harvest neoplastic stem cel s.OBJECTIVE:
To culture the cel clones of human neuroblastoma cel line SK-N-SH and to assess the biological properties of the cel clones.METHODS:
Using the suspension method with no serum media, tumor cel clones were obtained. Immunofluorescence method was used to identify whether tumor cel clones exhibit stem cel properties. SK-N-SH neuroblastoma was labeled by luciferase, and tumor cel clones and tumor cel s were seeded onto the back of nude mice to monitor cel proliferative properties in nude mice using in vivo imaging. RESULTS ANDCONCLUSION:
Using the suspension culture method, SK-N-SH neuroblastoma cel s could successful y develop into cloning bal s. Under serum-free culture, cloning bal s were immunofluorescently used to detect molecular markers that showed strong positive expression. Cloning bal s subcutaneously implanted into nude mice showed the strong ability of self-renewal and differentiation as stem cel s. The cel clones cultured by the suspension method strongly expressed Nestin, but weakly expressed glial fibril ary acidic protein, neuron-specific tubulin, possessing stem cel characteristics and strong proliferation and metastasis in nude mice.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2014
Tipo del documento:
Artículo
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