Isolation,purification and culture in vitro of stromal cells of human endometrium / 重庆医学

ABSTRACT

Objective To isolate,purify human endometrial stromal cells,and culture these cells in vitro,for the further research of pathogenesis of intrauterine adhesions.Methods Human endometrial stromal cells were separated and purificated by enzymo ly-sis,mesh filtration and pasted wall,then the stromal cells of purification were cultured.Phenotype of stromal cells was identified by immunohistochemistry.Results The stromal cells became adherent 30 min after culture.Two types of stromal cells were found. One was spindle and the other was polygonal.Immunocytochemical staining method revealed that vimentin was positive in both types of stromal cells,and the positive rate was above 9 5%;meanwhile,cytokeratin was negative in both types of stromal cells, which indicated that these cells were endometrial stromal cells.The isolated endometrial stromal cells can be cultured in vitro.Con-clusion Highly purified stromal cells of human endometrium could be obtained by enzymolysis,mesh filtration and pasted wall pur-ification,and the cultured stromal cells could passage to limited generations.