Age-associated proliferation and differentiation changes of rat bone marrow mesenchymal stem cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 18-23, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-460901
ABSTRACT
BACKGROUND:
Autologous bone marrow mesenchymal stem cel transplantation for the treatment of cardiovascular disease has become one of the hotspots, but it is unclear whether the proliferation and directional differentiation of autologous bone marrow mesenchymal stem cels varies changes with age.OBJECTIVE:
To explore the proliferation and differentiation changes of rat bone mesenchymal stem cels in different ages.METHODS:
The bone marrow mesenchymal stem cels from Sprague-Dawley rats in different age groups were purified and cultured, and then examined by flow cytometry in terms of cel cycle. Meanwhile, neonatal rat cardiomyocytes were co-cultured with bone marrow mesenchymal stem cels. The morphologic changes of bone marrow mesenchymal stem cels and the protein expression of troponin T were detected with immunofluorescence technique. RESULTS ANDCONCLUSION:
The percentage of bone marrow mesenchymal stem cels in G0/G1 phase was increased with age; while the percentage of expression of troponin T proteins-positive bone marrow mesenchymal stem cels were decreased with age. These findings indicate that the proliferation and differentiation abilities of rat bone marrow mesenchymal stem cels descend with age.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2015
Tipo del documento:
Artículo
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