Research of Cryoablation Minimally Invasive Lung Volume Reduction Surgery to Rabbit Emphysema Model / 医学研究杂志

ABSTRACT

Objective To perform the percutaneous Argon-Helium cryoablation on experimental emphysema rabbits Model, in or-der to eveluate the Percutaneous cryoablation of lung volume reduction surgery( LRVS) effectiveness, feasibility and safety.Methods The emphysema model were established on 20 healthy adult new Zealand white rabbits.The lung function and blood gas analysis were measured before and after the emphysema model establishment.After the emphysema model was established, the rabbit were punctured by CT-guided for Argon-helium cryoablation, which was performed for 2 cycles;frozen process for 5 minutes each time;helium active tha-wing, for 2 minutes each time.The result of lung function and blood gas analysis were measured on 1,3,7,14,28 days respectively, meanwhile obtain the corresponding specimens to observe the general morphological and histological changes under light microscopy.Re-sults During the Argon-Helium cryoablation of lung volume reduction surgery, 3 cases of mild intraoperative bleeding ( 15%) and 2 cases of subcutaneous emphysema (10%) happened.compared with emphysema model, lung function and blood gas analysis of the model did not change significantly 1 day post operation(P>0.05), on 3 days and 7 days post operation , lung function and blood gas analysis changed significantly (P<0.05);the lung function and blood gas analysis on 14 days, 28 days post operation have change significantly ( P<0.01) against emphysema model, the best time to improve the result of lung function and blood gas analysis is between 7-14 days. Conclusion Percutaneous cryoablation of lung volume reduction surgery achieve the purpose of effectively destroy the target tissue, sig-nificantly improve the animal model of pulmonary function and blood gas analysis index, with small trauma, fewer complications, higher efficacy and safety.