Simultaneous Determination of Chlorogenic Acid and Luteoloside in the Leaves of“Wudang No.II”Flos Lonicerae by HPLC-DAD / 医药导报

ABSTRACT

Objective To establish a HPLC method for simultaneous determination of chlorogenic acid and luteoloside in the leaves of“Wudang No.II” flos lonicerae. Methods Phenomenex C18(4. 6 mmí250 mm, 5μm) was used;the mobile phase was acetonitrile( A) and 0. 4% phosphoric acid aqueous solution( B) by gradient elution mode; the detection wavelength was 350 nm and the flow rate was 0. 8 mL·min-1;the column temperature was set at 32℃. Results The calibration curve of chlorogenic acid and luteoloside was linear in the range of 0. 285-2. 280μg(r=0. 999 3), and 0. 124-1. 240μg(r=0. 999 4), respectively. The mean recovery of chlorogenic acid and luteoloside was 98. 9%, RSD=1. 59% and 98. 8%, RSD=1. 84%, respectively. Conclusion This method was found to be accurate, quick and reproducible. It can be used for simultaneous determination of chlorogenic acid and luteoloside in the leaves of “Wudang NO.II”flos lonicerae.