Basic Fibroblast Growth Factor-Chitosan Carriers Induce Neural Stem Cells to Differentiate into Neurons and Form Synapses / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 406-411, 2015.
Article
en Zh
| WPRIM
| ID: wpr-465551
Biblioteca responsable:
WPRO
ABSTRACT
Objective To explore the effect of basic fibroblast growth factor (bFGF)-chitosan carriers on neural differentiation of neural stem cells (NSCs). Methods NSCs were isolated from spinal cord of a neonatal Wistar rat and cultured. Purity of cultured NSCs was identi-fied with Nestin immunofluorescent staining. The 10 mg/ml chitosan carriers, 20 ng/ml bFGF or 10 mg/ml bFGF-chitosan carriers were add-ed into medium of P3~P4 NSCs respectively. NSCs were observed with immunofluorescent staining: 3 days after incubation with Nestin andβ-tubulin III;7 days after incubation with microtubule-associated protein-2 (MAP2), glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP);and 14 days after incubation with synapsin-1 and MAP2. The electrophysiological activity of cells was detected with MED64. Results 3 days after incubation, all the NSCs differentiated into Nestin+/β-tubulin III+, and the length of neurofilament was the high-est in those co-cultured with bFGF-chitosan carriers. 7 days after incubation, NSCs differentiated into MAP2+, GFAP+and MBP+, and more NSCs differentiated into MAP2+with bFGF-chitosan carriers. 14 days after incubation, NSCs differentiated with bFGF-chitosan carriers ex-press synapsin-1+/MAP2+and showed electrophysiological activity. Conclusion bFGF-chitosan carriers can induce NSCs to differentiate in-to neuron with high percentage and the differentiated neurons can form synapses with electrophysiology activity.
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1
Índice:
WPRIM
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Rehabilitation Theory and Practice
Año:
2015
Tipo del documento:
Article