In vitro cytocompatibility of biomedical (Y0.95Eu0.05)2O3 nanomaterials / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1846-1851, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-465640
ABSTRACT
BACKGROUND:
Compared with organic fluorescent dyes and fluorescent proteins, rare earth nanomaterials have higher sensitivity, better optical stability, and lower cytotoxicity.OBJECTIVE:
To investigate thein vitro cytocompatibility of (Y0.95Eu0.05)2O3METHODS:
(Y rare earth nanomaterial and the effect of amine functionalization on the material cytocompatibility. 0.95Eu0.05)2O3 nanomaterials were synthesized using sol-gel method, and underwent amine functionalization. Amine-functionalized samples (10, 25, 50, 100, 200 mg/L) and non-amine-functionalized (Y0.95Eu0.05)2O3 rare earth nanomaterial suspensions were co-cultured with vascular smooth muscle cels of Sprague-Dawley rats for 3 days. Cel proliferation was detected using Cel Counting Kit-8 assay. 100 mg/L amine-functionalized samples and non-amine-functionalized (Y0.95Eu0.05)2O3 rare earth nanomaterial suspensions were co-cultured with L929 cels for 48 hours. Cel apoptosis was observed using fluorescence staining. RESULTS ANDCONCLUSION:
With increased mass concentration of material suspension, survival rate of vascular smooth muscle cels was gradualy reduced. At the mass concentration of 10, 25, 50 mg/L, cel survival rate was significantly higher in the amine-functionalized group than in the non-amine-functionalized group (P < 0.05). When the mass concentration of materials reached 200 mg/L, the cel survival rate decreased to 76% in the non-amine-functionalized group, but it was stil above 80% in the amine-functionalized group, showing significant differences between the two groups (P < 0.05). The growth of L929 cels was good and the number of apoptotic cels was less in the amine-functionalized group. Cels were sparse and the number of cel apoptosis was more in the non-amine-functionalized group, showing slight cytotoxicity. These data verified that amine-functionalization can improve cytocompatibility of (Y0.95Eu0.05)2O3 rare earth nanomaterial.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2015
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS