Effects of simvastatin on neurons autophagy in hippocampus following intrac-erebral hemorrhage in rats / 中国免疫学杂志

ABSTRACT

Objective:To evaluate effect of simvastatin on neurons autophagy in the hippocampus following intracerebral hemorrhage( ICH) in rats.Methods:All rats were divided randomly into sham group;ICH group and SIM group.The rat model of ICH was made by injection of Ⅶ type collagenase.The behavioral changes were evaluated with Garcia′s neurologic deficit score.Wet-dry weight method was used to evaluate brain edema.Additionally,localization of LC3 protein was determined by immunohistochemistry, and the expression of Beclin-1 in hippocampus was tested by Western blot methods.Results: Compared with ICH group,simvastatin treatment after ICH significantly improved neurologic deficit score ( P<0.05 ) , reduced the water content of brain tissue ( P<0.05 ) , inhibited the expression of LC3 and Beclin-1 protein(P<0.05).Conclusion:Our findings indicated that administration of simvastatin had neuroprotective effects in ICH rat model,which could be involved in inhibiting activation of autophagy in the hippocampus,finally reducing brain edema and neurobehavioral deficit.