Reversal effect study of vitamin D on leukemia multidrug resistance cells / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the reversal effect of vitamin D on drug resistance leukemia cell lines Jurcat vitamin D/ADR,K562/ADR and its mechanism.Methods Non-toxic concentration of vitamin D was chosen by methyl thiazolyl tetrazolium,and on line concentration experiment ;the contents of vitamin D for soft erythromycin in the cell and the cell surface of P-glycoprotein(P-gp) expression level were detected by using flow cytometry instrument and vitamin D for intracellular mdr1 mRNA and mrp1 mRNA expression was detected by Real-time polymerase chain reaction;vitamin D for the change of intra cellular glutathione glycosides peptide (GSH) content was detected by adoping biochemistry method.Results Resistance reversal in experiments showed that vitamin D could decrease soft erythromycin in the accumulation of drug resistant cells,with a dose-dependent relationship; vitamin D treatment Jurcat/ADR,K562/ADR cells after 48 h,chemotherapy drugs could increase the cell concentration,and reduce P-gp and the expression of mdr1 and mrp1 gene ; vitamin D treatment Jurcat/ADR,K562/ADR after 48 h could decrease the intracellular GSH content in K562,K562/ADR,Jurcat and Jurcat/ADR.Conclusions Vitamin D has the drug resistance reversal agents to Jurcat/ADR,K562/ADR,which might be relevant to the inhibition of cell surface of P-gp proteins function and expression and reduction of the mdr1 and mrp1 expressions of resistance genes and reduction of intracellular GSH content.Vitamin D by influencing the above mechanism,which increases the drug concentration in cells,can effectively kill the tumor cells.