Efficacy of Polymerase Chain Reaction in Early Diagnosis of Herpes Simplex Keratitis in Rabbit Model
Journal of the Korean Ophthalmological Society
;
: 137-144, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-46851
ABSTRACT
We evaluated the efficacy of polymerase chain reaction(PCR)in early diagnosis of herpes simplex keratitis(HSK)in rabbit model and compared the sensitivity and specificity of PCR in two kinds of primers. Only one eye of 8 rabbits was inoculated with HSV Type 1(F strain, ATCC VR-73)to induce HSK, and the other eye was used as control. Rabbit cornea was examined under slit lamp examination and PCR test of tear and corneal scraping specimens were performed at 1, 3, 5, 7, and 10th post-inoculation day. The sensitivity of PCR with POL primer was 100%in tear and corneal scraping specimens. The sensitivity of PCR with POL primer and LAT primer were 80%and 100%respectively. PCR test is very useful diagnotic tool for the early diagnosis of HSK in rabbit model. In addition, PCR test with corneal scraping specimens provided better yield compared with tears.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Sensibilidad y Especificidad
/
Queratitis Herpética
/
Córnea
/
Diagnóstico Precoz
/
Herpes Simple
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
2001
Tipo del documento:
Artículo
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