Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction / 소아과
Korean Journal of Pediatrics
;
: 424-429, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-47230
ABSTRACT
PURPOSE:
Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae-positive results.METHODS:
Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique.RESULTS:
Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable.CONCLUSION:
As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neumonía
/
Infecciones del Sistema Respiratorio
/
Streptococcus
/
Streptococcus pneumoniae
/
Serotipificación
/
Reacción en Cadena de la Polimerasa
/
Técnicas de Cultivo
/
Reacción en Cadena de la Polimerasa Multiplex
/
Corea (Geográfico)
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Korean Journal of Pediatrics
Año:
2012
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS