Construction of Packaging Plasmid pSNAV-hEndostatin-CMV-EGFP of AAV Encoding Human Endostatin cDNA / 天津医药
Tianjin Medical Journal
;
(12): 535-537, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-472914
ABSTRACT
Objective:
To construct the packaging plasmid pSNAV-hEndostatin-CMV-EGFP of adeno-associated virus vector (AAV) encoding human Endostatin (hEndostatin) cDNA.Methods:
The hEndostatin eDNA obtained from plasmid pCD-sEndostatin was subcloned into the packaging plasmid pSNAV of AAV by molecular clone ways. The recombinant plasmid pSNAV-hEndostatin-CMV-EGFP was identified by PCR analysis, restriction enzymes analysis and sequencing analysis.Results:
The recombinant pSNAV-hEndostatin-CMV-EGFP was correctly constructed and confirmed by PCR analysis, restriction enzymes analysis and sequencing analysis.Conclusion:
The constructed AAV-hEndostatin packaging plasmid pSNAV-hEndostatin-CMV-EGFP could be the packaging plasmid of rAAV-hEndostatin-EGFP.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Tianjin Medical Journal
Año:
2009
Tipo del documento:
Artículo
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