Chemiluminescence determination of melamine with LuminoI-K3Fe(CN)6 system / 药物分析学报
Journal of Pharmaceutical Analysis
; (6): 104-107, 2011.
Article
en Zh
| WPRIM
| ID: wpr-473267
Biblioteca responsable:
WPRO
ABSTRACT
A sensitive chemiluminescence(CL) method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe(CN)6 system in alkaline medium.The determination conditions were optimized.Under optimum conditions,the chemiluminescence intensity had a good linear relationship with melamine in the range of 9.0 × 10 97.0 × 10 6 g/mL with a correlation coefficient of 0.9992.The detection limits (3σ) were 3.54 ng/mL for urine sample and 6.58 ng/mL for plasma sample.The average recoveries of melamine were 102.6% for urine sample and 95.1% for plasma sample.Melamine in samples was extracted with liquid-liquid extraction procedures and the assay results coincided very well with that determined with flow injection chemiluminescence method.The method provides a reproducible and stable approach for sensitive detection and quantification of melamine in urine and plasma samples.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Analysis
Año:
2011
Tipo del documento:
Article