18F-FDG effects on maturation and immune function of dendritic cells in vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 6758-6762, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-475385
ABSTRACT
BACKGROUND:
Previous studies have shown that different doses of ionizing radiation may have some impact on maturation and immune function of dendritic cells.OBJECTIVE:
To evaluate the effect of 18 F-FDG with different concentrations on maturation and immune function of dendritic cells from human peripheral blood mononuclear cells in vitro.METHODS:
Human peripheral blood mononuclear cells-derived mature dendritic cells were divided into five groupsPBS, 92.5×10 4, 185×104, 370×104, 740×104 Bq/mL 18 F-FDG were separately added into each group. Dendritic cells were col ected 24 hours later. Apoptosis rate, phenotypic expression (CD1α, CD80, CD83, CD86, HLA-DR), ability of mixed lymphocyte reaction and expression of macrophage inflammatory protein 1αand monocyte chemotactic factor-1 in cellculture supernatant were detected. RESULTS ANDCONCLUSION:
Apoptosis rate, phenotypic expression of CD86, ability of mixed lymphocyte reaction and expression of monocyte chemotactic factor-1 were down-regulated after culture in 740×10 4 Bq/mL 18F-FDG. However, 18F-FDG at other concentrations had no influence on maturation and immune function of dendritic cells. This study demonstrates that low-concentration 18F-FDG has little influence on apoptosis, maturation, antigen presentation, and migratory capacity of dendritic cells, and it may be selected at an appropriate concentration as a label for dendritic cells in vitro.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2014
Tipo del documento:
Artículo
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