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18F-FDG effects on maturation and immune function of dendritic cells in vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53): 6758-6762, 2014.
Artículo en Chino | WPRIM | ID: wpr-475385
ABSTRACT

BACKGROUND:

Previous studies have shown that different doses of ionizing radiation may have some impact on maturation and immune function of dendritic cells.

OBJECTIVE:

To evaluate the effect of 18 F-FDG with different concentrations on maturation and immune function of dendritic cells from human peripheral blood mononuclear cells in vitro.

METHODS:

Human peripheral blood mononuclear cells-derived mature dendritic cells were divided into five groupsPBS, 92.5×10 4, 185×104, 370×104, 740×104 Bq/mL 18 F-FDG were separately added into each group. Dendritic cells were col ected 24 hours later. Apoptosis rate, phenotypic expression (CD1α, CD80, CD83, CD86, HLA-DR), ability of mixed lymphocyte reaction and expression of macrophage inflammatory protein 1αand monocyte chemotactic factor-1 in cellculture supernatant were detected. RESULTS AND

CONCLUSION:

Apoptosis rate, phenotypic expression of CD86, ability of mixed lymphocyte reaction and expression of monocyte chemotactic factor-1 were down-regulated after culture in 740×10 4 Bq/mL 18F-FDG. However, 18F-FDG at other concentrations had no influence on maturation and immune function of dendritic cells. This study demonstrates that low-concentration 18F-FDG has little influence on apoptosis, maturation, antigen presentation, and migratory capacity of dendritic cells, and it may be selected at an appropriate concentration as a label for dendritic cells in vitro.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Tissue Engineering Research Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Tissue Engineering Research Año: 2014 Tipo del documento: Artículo