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Microfluidic chip digital PCR technology and its clinical application / 中华检验医学杂志
Chinese Journal of Laboratory Medicine ; (12): 592-594, 2015.
Artículo en Chino | WPRIM | ID: wpr-479296
ABSTRACT
Microfluidics-based digital PCR depends on microfluidic chip to split PCR reaction mixture into many tiny equal-volume units.Quantitative assessment of target DNA template can be obtained by counting the number of fluorescence-positive units after thermocycling.Microfluidics-based digital PCR exhibits many advantages including absolute quantification, high sensitivity and accuracy, and shows great promise in a variety of applications, such as infectious diseases diagnose, early cancer detection and prenatal diagnose. There are already several microfludics-based digital PCR products produced from sereval companies.It is believed that as the technology improves, microfluidics-based digital PCR will find broader applications and become the next-generation tool for genetic tests.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio de tamizaje Idioma: Chino Revista: Chinese Journal of Laboratory Medicine Año: 2015 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio de tamizaje Idioma: Chino Revista: Chinese Journal of Laboratory Medicine Año: 2015 Tipo del documento: Artículo