The unduced effect of arsenic trioxide in different types of neuroblastoma cell lines / 中华实用儿科临床杂志
Chinese Journal of Applied Clinical Pediatrics
; (24): 1164-1167, 2015.
Article
en Zh
| WPRIM
| ID: wpr-480145
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WPRO
ABSTRACT
Objective To identify the effect of arsenic trioxide (As2O3) on the differentiation and apoptosis of different types of neuroblastoma(NB) cell lines.Methods The cell lines [SK-N-SH,SK-N-BE2,SH-SY5 Y] were induced with different concentrations (0 μmol/L,1 μmol/L,3 μmol/L,5 μ mol/L and 7 μ mol/L) of arsenic trioxide for 24 h,48 h,72 h under the same conditions.The expression of MYCN gene was examined by fluorescence in situ hybridization assay in SK-N-BE2,cell proliferation,cell cycle and cell apoptosis were detected with cell counting kit-8 (CCK-8) assay and flow cytometry.Results 5 μmol/L of As2O3 inhibited the expression of MYCN gene in SK-N-BE2;CCK-8 assay indicated that As2O3 inhibited the proliferation of NB cell in a dose-and time-dependent manner,the cell proliferation was significantly suppressed compared with the low concentration (1 μ mol/L) after treated with As2O3 by 1 μmol/L,3 μmol/L,5 μmol/L and 7 μmol/L in 24 h,48 h and 72 h,SH-SY5Y:24 h(chisq =9.666 7,P < 0.05),48 h (chisq =9.666 7,P < O.05),72 h (chisq =9.512 8,P < 0.05);SK-N-SH,24 h (chisq =10.38,P<0.054 6),48 h(chisq=8.641 0,P<0.05),72 h(chisq=9.461 5,P<0.05);SK-N-BE2:24 h (chisq =8.435 9,P <0.05),48 h(chisq =8.641 O,P <0.05),72 h(chisq =9.545 5,P <0.05);compared with the control group,the As2O3-treated cells showed increased apoptosis percentage,with the percentage of 1.6% (0 μmol/L),3.8% (1 μmol/L),6.1% (3 μmol/L),10.4% (5 μmol/L),40.2% (7 μ mol/L);the cell cycle was arrested at G2/M phase,which prevented cell division.Conclusions (1) As2O3 play an important role on the NB cells proliferation,apoptosis which were dose-and time-dependent manner.(2)As2O3 can inhibit the expression of MYCN gene.(3)As2O3 also could block NB cell cycle at S and G2/M,and inhibit the cell nucleus replication and the As2O3 had different induced effect between different types of NB cell.
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Idioma:
Zh
Revista:
Chinese Journal of Applied Clinical Pediatrics
Año:
2015
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Article