Preliminary structural identification and protection on renal cell injury of acidic polysaccharide from Cordyceps sinensis / 中国药科大学学报

ABSTRACT

Aim: To elucidate the structure of an acidic polysaccharide from Cordyceps sinensis and to study its protective effect on renal cell injury by cisplatin. Methods: After Cordyceps sinensis acidic polysaccharide( CAPS) was isolated and purified, the purity and molecule weight were detected by high performance gel permeation chro-matography( HPGPC). Based on the UV spectrum, FT-IR spectrum, monosaccharide composition and GC-MS, the structures were elucidated. Cisplatin-induced injury of Madin-Darby canine kidney( MDCK) cell line was protec-ted by the blood serum with CAPS. Results: The purity of CAPS, with a molecule weight of 2. 7×10~4, was about 99%. It consisted mainly of mannose, glucose and galactose. When the serum with CAPS reached 2.5%, it showed difference compared with the cisplatin group (P <0. 05) on the protective effect of renal cell injury. It showed significant difference( P <0.01) when the serum with CAPS exceeded 5%. Conclusion: CAPS has a pro-tective effect on the renal cell injury in vitro.