An Approach to Diagnosing Gastrointestinal Stromal Tumors Using Immunohistochemistry of c-kit and PDGFRA with Molecular Analysis
Korean Journal of Pathology
;
: 173-178, 2010.
Artículo
en Coreano
| WPRIM
| ID: wpr-48174
ABSTRACT
BACKGROUND:
Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors in the gastrointestinal tract. Recently, many methods for the diagnosis of GIST have been developed including molecular diagnosis.METHODS:
We selected 90 cases of GIST that had presented at Kyungpook National University Hospital between 1998 and 2007. Tissue microarrays were made using core areas of tumor tissues. Immunohistochemical staining for c-kit, protein kinase C-theta, and platelet-derived growth factor receptor alpha (PDGFRA) was done. Direct sequencing of hot spot exonal areas for c-kit and PDGFRA were done using extracted DNAs of all 90 paraffin block tissues.RESULTS:
Among the 90 cases, 83.3% (75/90) were c-kit positive, 16.6% (15/90) were c-kit negative, 93.3% (84/90) were PDGFRA positive, and 6.6% (6/90) cases were PDGFRA negative. Fifteen cases of c-kit negative GIST included 1 case of PDGFRA negative and 5 cases of PDGFRA negative GIST were ckit positive. The one case in which both c-kit and PDGFRA were negative, showed a c-kit mutation in exon 11.CONCLUSIONS:
Combined immunohistochemical staining of c-kit, discovered on GIST 1 (DOG1) and PDGFRA is helpful for the diagnosis of GIST. When all staining tests are negative for immunoreactivity, c-kit mutation analysis for exon 11, 9 should be done. Genotyping of kit and PDGFRA do not need to be examined initially, if it is only for the diagnosis of GIST.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Parafina
/
Proteínas Quinasas
/
ADN
/
Inmunohistoquímica
/
Exones
/
Análisis de Secuencia
/
Receptores del Factor de Crecimiento Derivado de Plaquetas
/
Tracto Gastrointestinal
/
Tumores del Estroma Gastrointestinal
Tipo de estudio:
Estudio diagnóstico
Idioma:
Coreano
Revista:
Korean Journal of Pathology
Año:
2010
Tipo del documento:
Artículo
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